Review



e0554 jetprime polyplus  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    New England Biolabs e0554 jetprime polyplus
    E0554 Jetprime Polyplus, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5716 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e0554+jetprime+polyplus/Q5+Site-Directed+Mutagenesis+Kit/pm40434888-256-60-58
    Average 99 stars, based on 5716 article reviews
    e0554 jetprime polyplus - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Magnetic Beads:

    Article Title: A genome-wide base-editing screen uncovers a pivotal role of paxillin δ ubiquitination in influenza virus infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PB2 pAb GeneTex Cat#: GTX125926; RRID:AB_11162999 Rabbit anti-PA pAb GeneTex Cat#: GTX118991; RRID:AB_10619959 Rabbit anti-HA pAb GeneTex Cat#: GTX127357; RRID:AB_2728683 Rabbit anti-M1 pAb GeneTex Cat#: GTX636675; RRID:AB_2910042 Mouse anti-NP mAb Merck Millipore Cat#: MAB8251; RRID:AB_95293 Mouse anti-M2 mAb Santa Cruz Biotechnology Cat#: sc-32238; RRID:AB_627808 Rabbit anti-Paxillin pAb Cell Signaling Technology Cat#: 12065; RRID:AB_2797814 Cat#: 50195 Mouse anti-β Actin mAb TransGen Biotech Cat#: HC201-01 Mouse anti-Flag mAb TransGen Biotech Cat#: HT201-01 Mouse anti-HA mAb TransGen Biotech Cat#: HT301-01 Rabbit anti-GAPDH pAb Sigma-Aldrich Cat#: SAB4300645; RRID:AB_11127603 Rabbit anti-EEA1 mAb ABclonal Technology Cat#: A5057 Alexa Fluor TM 488 Goat anti-mouse IgG (H + L) ZSGB-BIO Cat#: ZF-0512; RRID:AB_3662677 Alexa Fluor TM 594 Goat anti-Rabbit IgG (H + L) ZSGB-BIO Cat#: ZF-0516; RRID:AB_2936330 Bacterial and virus strains Influenza A/WSN/1933 (H1N1) Prof. Ervin Fodor, University of Oxford, UK N/A Influenza A/Puerto Rico/8/1934 (H1N1) Prof. Ervin Fodor, University of Oxford, UK N/A Influenza A/Quail/Hong Kong/G1/1997 (H9N2) Dr Chunfeng Li, Institute of Biophysics of the Chinese Academy of Sciences, China N/A Influenza A/Viet Nam/1203/2004, VN04-HALo (H5N1) Prof Adolfo Garcı́a-Sastre, Icahn School of Medicine at Mount Sinai, USA N/A Vesicular stomatitis virus (VSV) This paper N/A GFP-encoding Newcastle Disease Virus (NDV-GFP) This paper N/A Respiratory Syncytial Virus (RSV) This paper N/A Sendai Virus (SeV) This paper N/A Escherichia coli strain DH5a Tsingke Cat#: TSC-C01 Escherichia coli strain Stbl3 TransGen Biotech Cat#: CD521-01 Chemicals, peptides, and recombinant proteins 3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4sulfophenyl)-2H-tetrazolium (MTS) Promega Cat#: G109A Phenazine methosulfate (PMS) Promega Cat#: G110A RIPA Lysis Buffer Beyotime Biotechnology Cat#: P0013B cOmplete protease inhibitor cocktail Roche Cat#: 11836170001 Cycloheximide (CHX) Sigma-Aldrich Cat#: 239763 4 ′ , 6-diamidino-2-phenylindole dihydrochloride (DAPI) Sigma-Aldrich Cat#: D9542 Geneticin (G418) MedChemExpress Cat#: HY-K1056 Puromycin Dihydrochloride Solarbio Cat#: P8230 Doxycycline (Dox) MedChemExpress Cat#: HY-N0565 (Continued on next page) Cell Reports 44, 115748, June 24, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER anti-Flag M2 magnetic beads Sigma-Aldrich Cat#: M8823 DMEM Life technologies Cat#: C11995500BT Opti-MEM Life technologies Cat#: 31985070 DMEM/F12 Life technologies Cat#: C11330500BT T4 DNA Ligase New England Biolabs Cat#: M0202 T4 Polynucleotide Kinase New England Biolabs Cat#: M0201 Critical commercial assays DNeasy Blood and Tissue Kit Qiagen Cat#: 69506 Q5 Site-Directed Mutagenesis Kit NEB Cat#: E0554 jetPRIME PolyPlus Cat#: 101000001 Lipofectamine RNAiMAX Invitrogen Cat#: 13778150 TRIzol Invitrogen Cat#: 15596018 SuperScript III Invitrogen Cat#: 18080093 M-MLV Reverse Transcriptase Invitrogen Cat#: 28025013 PowerUpN SYBRN Green Thermo Fisher Cat#: A25742 Experimental models: Cell lines A549 ATCC Cat#: CCL-185 MDCK ATCC Cat#: PTA-6500 HEK293T ATCC Cat#: CRL-11268 hTERT-RPE1 Y. Sun’s laboratory, Peking University N/A hTERT-RPE1-ABEmax-SC This paper N/A Experimental models: Organisms/strains BALB/c mice Charles River Laboratories, Beijing, China N/A Oligonucleotides Paxillin-knockout sgRNA: 5 ′ -AGGGACGTGCTCATTACGGT-3 ′ This paper N/A Paxillin lysine mutate sgRNA: 5 ′ -GAGAAGCCTAAGCGGAATG-3 ′ This paper N/A PPMO targeting mouse paxillin #1: #1: 5 ′ -TGGTCCTCAGACGCCAGTCCCC-3 ′ This paper N/A PPMO targeting mouse paxillin #2: #2: 5 ′ -CTCTGCCGACTTCTGCTTGTTG-3 ′ This paper N/A PPMO-NC: 5 ′ -CCTCTTACCTCAGTTACAATTTATA-3 ′ This paper N/A 32 P-labelled primers forward: 5 ′ -TGGACTAGTGGGAGCATCAT-3 ′ Zhang et al. 57 https://doi.org/10.1128/jvi.00337-23. ..

    Mutagenesis:

    Article Title: A genome-wide base-editing screen uncovers a pivotal role of paxillin δ ubiquitination in influenza virus infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PB2 pAb GeneTex Cat#: GTX125926; RRID:AB_11162999 Rabbit anti-PA pAb GeneTex Cat#: GTX118991; RRID:AB_10619959 Rabbit anti-HA pAb GeneTex Cat#: GTX127357; RRID:AB_2728683 Rabbit anti-M1 pAb GeneTex Cat#: GTX636675; RRID:AB_2910042 Mouse anti-NP mAb Merck Millipore Cat#: MAB8251; RRID:AB_95293 Mouse anti-M2 mAb Santa Cruz Biotechnology Cat#: sc-32238; RRID:AB_627808 Rabbit anti-Paxillin pAb Cell Signaling Technology Cat#: 12065; RRID:AB_2797814 Cat#: 50195 Mouse anti-β Actin mAb TransGen Biotech Cat#: HC201-01 Mouse anti-Flag mAb TransGen Biotech Cat#: HT201-01 Mouse anti-HA mAb TransGen Biotech Cat#: HT301-01 Rabbit anti-GAPDH pAb Sigma-Aldrich Cat#: SAB4300645; RRID:AB_11127603 Rabbit anti-EEA1 mAb ABclonal Technology Cat#: A5057 Alexa Fluor TM 488 Goat anti-mouse IgG (H + L) ZSGB-BIO Cat#: ZF-0512; RRID:AB_3662677 Alexa Fluor TM 594 Goat anti-Rabbit IgG (H + L) ZSGB-BIO Cat#: ZF-0516; RRID:AB_2936330 Bacterial and virus strains Influenza A/WSN/1933 (H1N1) Prof. Ervin Fodor, University of Oxford, UK N/A Influenza A/Puerto Rico/8/1934 (H1N1) Prof. Ervin Fodor, University of Oxford, UK N/A Influenza A/Quail/Hong Kong/G1/1997 (H9N2) Dr Chunfeng Li, Institute of Biophysics of the Chinese Academy of Sciences, China N/A Influenza A/Viet Nam/1203/2004, VN04-HALo (H5N1) Prof Adolfo Garcı́a-Sastre, Icahn School of Medicine at Mount Sinai, USA N/A Vesicular stomatitis virus (VSV) This paper N/A GFP-encoding Newcastle Disease Virus (NDV-GFP) This paper N/A Respiratory Syncytial Virus (RSV) This paper N/A Sendai Virus (SeV) This paper N/A Escherichia coli strain DH5a Tsingke Cat#: TSC-C01 Escherichia coli strain Stbl3 TransGen Biotech Cat#: CD521-01 Chemicals, peptides, and recombinant proteins 3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4sulfophenyl)-2H-tetrazolium (MTS) Promega Cat#: G109A Phenazine methosulfate (PMS) Promega Cat#: G110A RIPA Lysis Buffer Beyotime Biotechnology Cat#: P0013B cOmplete protease inhibitor cocktail Roche Cat#: 11836170001 Cycloheximide (CHX) Sigma-Aldrich Cat#: 239763 4 ′ , 6-diamidino-2-phenylindole dihydrochloride (DAPI) Sigma-Aldrich Cat#: D9542 Geneticin (G418) MedChemExpress Cat#: HY-K1056 Puromycin Dihydrochloride Solarbio Cat#: P8230 Doxycycline (Dox) MedChemExpress Cat#: HY-N0565 (Continued on next page) Cell Reports 44, 115748, June 24, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER anti-Flag M2 magnetic beads Sigma-Aldrich Cat#: M8823 DMEM Life technologies Cat#: C11995500BT Opti-MEM Life technologies Cat#: 31985070 DMEM/F12 Life technologies Cat#: C11330500BT T4 DNA Ligase New England Biolabs Cat#: M0202 T4 Polynucleotide Kinase New England Biolabs Cat#: M0201 Critical commercial assays DNeasy Blood and Tissue Kit Qiagen Cat#: 69506 Q5 Site-Directed Mutagenesis Kit NEB Cat#: E0554 jetPRIME PolyPlus Cat#: 101000001 Lipofectamine RNAiMAX Invitrogen Cat#: 13778150 TRIzol Invitrogen Cat#: 15596018 SuperScript III Invitrogen Cat#: 18080093 M-MLV Reverse Transcriptase Invitrogen Cat#: 28025013 PowerUpN SYBRN Green Thermo Fisher Cat#: A25742 Experimental models: Cell lines A549 ATCC Cat#: CCL-185 MDCK ATCC Cat#: PTA-6500 HEK293T ATCC Cat#: CRL-11268 hTERT-RPE1 Y. Sun’s laboratory, Peking University N/A hTERT-RPE1-ABEmax-SC This paper N/A Experimental models: Organisms/strains BALB/c mice Charles River Laboratories, Beijing, China N/A Oligonucleotides Paxillin-knockout sgRNA: 5 ′ -AGGGACGTGCTCATTACGGT-3 ′ This paper N/A Paxillin lysine mutate sgRNA: 5 ′ -GAGAAGCCTAAGCGGAATG-3 ′ This paper N/A PPMO targeting mouse paxillin #1: #1: 5 ′ -TGGTCCTCAGACGCCAGTCCCC-3 ′ This paper N/A PPMO targeting mouse paxillin #2: #2: 5 ′ -CTCTGCCGACTTCTGCTTGTTG-3 ′ This paper N/A PPMO-NC: 5 ′ -CCTCTTACCTCAGTTACAATTTATA-3 ′ This paper N/A 32 P-labelled primers forward: 5 ′ -TGGACTAGTGGGAGCATCAT-3 ′ Zhang et al. 57 https://doi.org/10.1128/jvi.00337-23. ..

    Reverse Transcription:

    Article Title: A genome-wide base-editing screen uncovers a pivotal role of paxillin δ ubiquitination in influenza virus infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-PB2 pAb GeneTex Cat#: GTX125926; RRID:AB_11162999 Rabbit anti-PA pAb GeneTex Cat#: GTX118991; RRID:AB_10619959 Rabbit anti-HA pAb GeneTex Cat#: GTX127357; RRID:AB_2728683 Rabbit anti-M1 pAb GeneTex Cat#: GTX636675; RRID:AB_2910042 Mouse anti-NP mAb Merck Millipore Cat#: MAB8251; RRID:AB_95293 Mouse anti-M2 mAb Santa Cruz Biotechnology Cat#: sc-32238; RRID:AB_627808 Rabbit anti-Paxillin pAb Cell Signaling Technology Cat#: 12065; RRID:AB_2797814 Cat#: 50195 Mouse anti-β Actin mAb TransGen Biotech Cat#: HC201-01 Mouse anti-Flag mAb TransGen Biotech Cat#: HT201-01 Mouse anti-HA mAb TransGen Biotech Cat#: HT301-01 Rabbit anti-GAPDH pAb Sigma-Aldrich Cat#: SAB4300645; RRID:AB_11127603 Rabbit anti-EEA1 mAb ABclonal Technology Cat#: A5057 Alexa Fluor TM 488 Goat anti-mouse IgG (H + L) ZSGB-BIO Cat#: ZF-0512; RRID:AB_3662677 Alexa Fluor TM 594 Goat anti-Rabbit IgG (H + L) ZSGB-BIO Cat#: ZF-0516; RRID:AB_2936330 Bacterial and virus strains Influenza A/WSN/1933 (H1N1) Prof. Ervin Fodor, University of Oxford, UK N/A Influenza A/Puerto Rico/8/1934 (H1N1) Prof. Ervin Fodor, University of Oxford, UK N/A Influenza A/Quail/Hong Kong/G1/1997 (H9N2) Dr Chunfeng Li, Institute of Biophysics of the Chinese Academy of Sciences, China N/A Influenza A/Viet Nam/1203/2004, VN04-HALo (H5N1) Prof Adolfo Garcı́a-Sastre, Icahn School of Medicine at Mount Sinai, USA N/A Vesicular stomatitis virus (VSV) This paper N/A GFP-encoding Newcastle Disease Virus (NDV-GFP) This paper N/A Respiratory Syncytial Virus (RSV) This paper N/A Sendai Virus (SeV) This paper N/A Escherichia coli strain DH5a Tsingke Cat#: TSC-C01 Escherichia coli strain Stbl3 TransGen Biotech Cat#: CD521-01 Chemicals, peptides, and recombinant proteins 3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4sulfophenyl)-2H-tetrazolium (MTS) Promega Cat#: G109A Phenazine methosulfate (PMS) Promega Cat#: G110A RIPA Lysis Buffer Beyotime Biotechnology Cat#: P0013B cOmplete protease inhibitor cocktail Roche Cat#: 11836170001 Cycloheximide (CHX) Sigma-Aldrich Cat#: 239763 4 ′ , 6-diamidino-2-phenylindole dihydrochloride (DAPI) Sigma-Aldrich Cat#: D9542 Geneticin (G418) MedChemExpress Cat#: HY-K1056 Puromycin Dihydrochloride Solarbio Cat#: P8230 Doxycycline (Dox) MedChemExpress Cat#: HY-N0565 (Continued on next page) Cell Reports 44, 115748, June 24, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER anti-Flag M2 magnetic beads Sigma-Aldrich Cat#: M8823 DMEM Life technologies Cat#: C11995500BT Opti-MEM Life technologies Cat#: 31985070 DMEM/F12 Life technologies Cat#: C11330500BT T4 DNA Ligase New England Biolabs Cat#: M0202 T4 Polynucleotide Kinase New England Biolabs Cat#: M0201 Critical commercial assays DNeasy Blood and Tissue Kit Qiagen Cat#: 69506 Q5 Site-Directed Mutagenesis Kit NEB Cat#: E0554 jetPRIME PolyPlus Cat#: 101000001 Lipofectamine RNAiMAX Invitrogen Cat#: 13778150 TRIzol Invitrogen Cat#: 15596018 SuperScript III Invitrogen Cat#: 18080093 M-MLV Reverse Transcriptase Invitrogen Cat#: 28025013 PowerUpN SYBRN Green Thermo Fisher Cat#: A25742 Experimental models: Cell lines A549 ATCC Cat#: CCL-185 MDCK ATCC Cat#: PTA-6500 HEK293T ATCC Cat#: CRL-11268 hTERT-RPE1 Y. Sun’s laboratory, Peking University N/A hTERT-RPE1-ABEmax-SC This paper N/A Experimental models: Organisms/strains BALB/c mice Charles River Laboratories, Beijing, China N/A Oligonucleotides Paxillin-knockout sgRNA: 5 ′ -AGGGACGTGCTCATTACGGT-3 ′ This paper N/A Paxillin lysine mutate sgRNA: 5 ′ -GAGAAGCCTAAGCGGAATG-3 ′ This paper N/A PPMO targeting mouse paxillin #1: #1: 5 ′ -TGGTCCTCAGACGCCAGTCCCC-3 ′ This paper N/A PPMO targeting mouse paxillin #2: #2: 5 ′ -CTCTGCCGACTTCTGCTTGTTG-3 ′ This paper N/A PPMO-NC: 5 ′ -CCTCTTACCTCAGTTACAATTTATA-3 ′ This paper N/A 32 P-labelled primers forward: 5 ′ -TGGACTAGTGGGAGCATCAT-3 ′ Zhang et al. 57 https://doi.org/10.1128/jvi.00337-23. ..



    Similar Products

    e0554 jetprime polyplus  (New England Biolabs)
    99
    New England Biolabs e0554 jetprime polyplus
    E0554 Jetprime Polyplus, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e0554+jetprime+polyplus/Q5+Site-Directed+Mutagenesis+Kit/pm40434888-256-60-58
    Average 99 stars, based on 1 article reviews
    e0554 jetprime polyplus - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    e0554 jetprime polyplus  (Sartorius AG)
    99
    Sartorius AG e0554 jetprime polyplus
    E0554 Jetprime Polyplus, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e0554+jetprime+polyplus/jetPRIME+DNA%2FsiRNA/pm40434888-256-60-62
    Average 99 stars, based on 1 article reviews
    e0554 jetprime polyplus - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results